POOLED HUMAN SERUM

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Autologous versus pooled human serum for articular chondrocyte growth.

PURPOSE To evaluate the effect of autologous human serum (AHS) versus pooled human serum (PHS) versus foetal bovine serum (FBS) for growth of articular chondrocytes and formation of chondrocytefibrin constructs. METHODS Experiments with monolayer culture expansion of human articular chondrocytes were performed using basic culture media supplemented with 10% AHS, PHS, or FBS. Growth kinetics a...

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Immunoglobulin A Preparation from Human Pooled Plasma Using Plasma Fractionation and Ion Exchange Chromatography

Background: In this study we have prepared the IgA solution from normal human plasma using plasma fractionation and ion exchange chromatography. Materials and Methods: Using fractionation of plasma with cold ethanol (starting with 8% ethanol), fraction III was prepared as a suitable source for IgA preparation. Then it was treated with caprylic acid for separation of impurities. For enrichm...

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Preparation of pooled human platelet lysate (pHPL) as an efficient supplement for animal serum-free human stem cell cultures.

Platelet derived growth factors have been shown to stimulate cell proliferation efficiently in vivo(1,2) and in vitro. This effect has been reported for mesenchymal stromal cells (MSCs), fibroblasts and endothelial colony-forming cells with platelets activated by thrombin(3-5) or lysed by freeze/thaw cycles(6-14) before the platelet releasate is added to the cell culture medium. The trophic eff...

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Risk of jaundice following transfusion with pooled plasma or serum.

It has been independently shown by Spurling, Shone, and Vaughan (1946), and by Truelove and Hogben (1947), that the use ofpooled serum or plasma, in contradistinction to whole blood from individual donors, increases the risk of hepatitis following blood transfusion. This conclusion raises the elementary statistical problem: how does reduction of the number of contributors to a blood pool dimini...

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Pooled energy budget and human life history.

Human life history contains a series of paradoxes not easily explained by classical life history theory. Although overall reproductive output is higher than in related primates, juvenile growth is slower and age-specific reproductive rates decline faster with age. A simple energetic model would predict that growth and reproductive rates should be positively correlated and that reproductive effo...

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ژورنال

عنوان ژورنال: Annals of Surgery

سال: 1945

ISSN: 0003-4932

DOI: 10.1097/00000658-194503000-00008